Fig. 5
From: XPC is an RNA polymerase II cofactor recruiting ATAC to promoters by interacting with E2F1
Ménage-à-trois between XPC, KAT2A, and E2F1. a Diagrams representing the fragment depth of E2F1 from ENCODE ChIP-seq data for XPC-negatively regulated (green) and XPC-positively regulated (blue) genes, as they were previously determined in XP-CWT and XP-CDEL. b ChIP experiment investigating the occupancy of E2F1 at CCND1 and DAPK1 promoters, with or without ATRA treatment. Error bars represent the standard deviation of three independent experiments.c Immunoprecipitation performed on nuclear extracts from untreated XP-CWT fibroblasts with antibody against E2F1 or IgG. Western blot was revealed with antibodies directed against E2F1, XPC, and KAT2A. d Left panel; blue staining of recombinant Flag-KAT2A, duplex Strep-XPC/His-HR23B, and c-myc-E2F1. Right panel; in vitro co-immunoprecipitation assay performed by antibodies against Flag tag with the recombinant protein Flag-KAT2A, the duplex Strep-XPC/His-HR23B, and c-myc-E2F1
