Fig 7

PPC reflects choice independent of stimulus-reward contingency. a Mice were trained on a go/no-go discrimination task with a reversed reward contingency. b Behavioral performance before and after reversal. Left, response rate for Stimulus A (red) and Stimulus B (blue). Right, d-prime assuming Stimulus A as target. Performance after reversal was significantly different from zero (p < 0.001, Wilcoxon signed-rank test). c Neural response of two example V1 neurons before and after reversal of reward contingency. Colors indicate stimulus (red, A; blue, B) and line styles indicate whether the stimulus is target (solid) or non-target (dashed). Top row, response of a neuron selective to Stimulus A both before (left) and after (right) reversal. Bottom row, response of a neuron selective to Stimulus B. Insets indicate the average projection image before and after reversal, and ROI (dashed red line) that was used to extract the neural response. d Same as c but for two PPC neurons. These neurons show a switch in selectivity with reversal of reward contingency. e Histogram of selectivity index before and after reversal for V1 neurons with significant responses both before and after reversal (n = 488). Positive values indicate selectivity to the original Target, Stimulus A (red). Colored bars indicated neurons with significant selectivity. f Same as e but for PPC neurons (n = 509). g Scatter plot comparing selectivity index in V1 before and after reversal. Colored points indicate neurons with significant selectivity both before and after reversal. Neurons in the first and third quadrants are stimulus-selective, as they prefer Stimulus A (red) or Stimulus B (blue) both before and after reversal. Neurons in the second and third quadrants are choice-selective, as they prefer either the Go stimulus (green) or the No-Go stimulus (yellow) in both conditions. h Same as g, but for PPC