Fig. 7
TMEM106B drives invasion by inducing elevated production and release of cathepsins. a Activity of cathepsins were assessed using specific activity assays in conditioned phosphate buffered saline (conditioned PBS) where lysosomal contents were released by treating mouse, or b human cells expressing either GFP only or TMEM106B, briefly with ionomycin or DMSO (as control) in the presence of 2 mM Ca2+. Assays were also performed in the presence of specific inhibitors, as indicated. c Invasion assays performed with non-invasive 393P cells, which were incubated with conditioned PBS of human or mouse cells induced for either GFP (Vec) as control or TMEM106B, in the presence of either DMSO as control or a protease inhibitor cocktail. d Invasion assays performed with noninvasive 393P cells, which were incubated with conditioned PBS of human or mouse cells induced for either GFP (Vec) or TMEM106B, in the presence of either DMSO or the irreversible cysteine protease inhibitor (E64D), which specifically inhibits cathepsins B, H, and K
