Fig. 3
From: TCR signal strength controls thymic differentiation of iNKT cell subsets
Thymic iNKT subsets with different TCR levels express different proportions of Vβ usage but discrete Egr2 levels. Cells from the thymus of 8-week-old BALB/c mice were stained with anti-TCRβ mAbs, PBS57–CD1d tetramers, mAbs specific for the transcription factors PLZF, Rorγt, and Tbet and the indicated anti-Vβ mAbs. a iNKT cells were subdivided based on expression of different TCR levels. The proportion of iNKT cell subsets for each level of TCR expression (TCRlow (blue), TCRint (orange) and TCRhigh (green)) is shown. b The percentage of Vβ usage found in each iNKT subset expressing three distinct TCR levels is shown with the fluorescence minus one control (FMO). Data are representative of three independent mice analyzed. c Example of Egr2 expression in each iNKT cell subsets expressing different levels of TCR. d The gMFI of Egr2 expression was recorded from three independent staining of iNKT cell subsets expressing different TCR levels from one individual mouse as gated in a. Three independent mice were analyzed in this way with similar results
