Fig. 4
Kcnj13 orchestrates SM cell alignment and polarity. a Immunostaining for αSMA (red) and DAPI staining (blue) in dorsal views of E14.5 WT (n = 8) and Kcnj13T38C/T38C (n = 8) tracheas. b Quantification of E14.5 WT (n = 8) and Kcnj13T38C/T38C (n = 8) tracheal SM cell orientation. c Quantification of E14.5 WT (n = 8) and Kcnj13T38C/T38C (n = 8) tracheal SM cell nuclear aspect ratio (NAR). d Immunostaining for αSMA (red) and DAPI staining (blue) of transverse sections of E14.5 WT (n = 10) and Kcnj13T38C/T38C (n = 10) tracheas. e Quantification of WT (n = 10) and Kcnj13T38C/T38C (n = 10) tracheal SM cell layers. f Immunostaining for αSMA (red) and GM130 (green) and DAPI staining (blue) in dorsal views of E14.5 WT (n = 8) and Kcnj13T38C/T38C (n = 8) tracheas. g Quantification of WT (n = 8) and Kcnj13T38C/T38C (n = 8) Golgi apparatus (green) position relative to the nucleus (blue). h Timeline for tamoxifen administration. i Immunostaining for KCNJ13 (red), αSMA (green), and DAPI staining (blue) of transverse sections of E14.5 control (n = 6) and Myh11-CreERT2;Kcnj13 flox/flox (n = 6) tracheas. j Representative images of ventral views of E14.5 control (n = 9) and Myh11-CreERT2;Kcnj13 flox/flox (n = 9) tracheas. Double-sided arrows indicate tracheal tube length. k Quantification of E14.5 control (n = 9) and Myh11-CreERT2;Kcnj13 flox/flox (n = 9) tracheal tube length. l Immunostaining for αSMA (red) and DAPI staining (blue) in dorsal views of E14.5 control (n = 9) and Myh11-CreERT2;Kcnj13 flox/flox (n = 9) tracheas. m Quantification of E14.5 control (n = 9) and Myh11-CreERT2;Kcnj13 flox/flox (n = 9) tracheal SM cell orientation. n Immunostaining for SOX9 (green) in ventral views of E15.5 control (n = 6) and Myh11-CreERT2;Kcnj13 flox/flox (n = 6) tracheas. Arrows point to tracheal mesenchymal condensations. Scale bars: 1000 μm (j), 100 μm (n), 50 μm (d), 20 μm (a, f, i, l). *P < 0.05; **P < 0.01; unpaired Student’s t-test, mean ± s.d
