Fig. 5

Reduced abundance of mitochondrial proteins in C9ALS iPSCs. a Of the oxidative phosphorylation proteins detected by MS (n = 61), 52% were significantly reduced in C9ALS iPSCs, and 16% were similarly reduced in sALS iPSCs compared to controls (Cntl). b Fold change in each of 61 oxidative phosphorylation proteins. c Cumulative change in the abundance of oxidative phosphorylation proteins in ALS iPSCs. d 45% and 3% of the 95 cytoplasmic ribosomal proteins detected by MS in ALS iPSCs were significantly increased in C9ALS and sALS iPSCs, respectively, compared to controls. e Fold change for the 95 cytoplasmic ribosomal proteins in ALS iPSCs. f Cumulative change in the abundance of cytoplasmic ribosomal proteins in ALS iPSCs. g 72 separate mitochondrial ribosomal proteins were detected by MS in ALS iPSCs; 69% and 3% were reduced in abundance in C9ALS and sALS iPSCs, respectively. h Fold change for the 72 mitochondrial ribosomal proteins in ALS iPSCs. i Cumulative change in the abundance of mitochondrial ribosomal proteins in ALS iPSCs. FDR false discovery rate. All experiments performed in triplicate, with two lines /condition. Plots in c, f, i show mean ± standard deviation. *p < 0.0001; #p < 0.05, one-way ANOVA with Benjamini−Hochberg correction for multiple observations