Fig. 4
From: Rapid transport of deformation-tuned nanoparticles across biological hydrogels and cellular barriers
NP entry into tissues covered with hydrogels in vivo. a Distribution of NPs in rat intestines. Intestinal ligated loops were incubated with DiI-labeled NPs for 1 h. Fluorescence micrographs were obtained from transverse cryo-sections of the intestines. “L” indicates the lumen of the intestine. Blue: nuclei of the intestinal villi. Red: NPs. b In vivo imaging of BxPC-3 and HPSC tumor-bearing mice taken at the indicated time points before and after peritumoral injection of IR783-labeled soft, semi-elastic, or hard NPs. c Distribution of DiI-labeled softer, semi-elastic, and harder NPs in tumor slices of BxPC-3 and HPSC tumor xenografts 6 h after peritumoral injection. The cell nuclei were counterstained with DAPI. The tumor vessels were labeled with anti-CD31 antibody. Scale bars: 50 μm. Softer: Lip-F1275% NPs; Semi: PLGA70-Lip-F1275% NPs; Harder: PLGA160-Lip-F1275% NPs. (n = 3)
