Fig. 2
From: Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
Second round of chemical optimization of CRISPR RNAs. The optimized crRNA designs are shown in a, whereas chemical designs of tracrRNA are shown in b. Each crRNA was tested with the unmodified tracrRNA T0, whereas each tracrRNA was tested with the unmodified crRNA C0. The bar graphs show the percent of cells expressing mCherry, ±SD. Each RNA was tested in triplicate
