Table 1 Chemically modified crRNAs used in this study
From: Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
Guide | Repeat | |
|---|---|---|
C1 | GGUGAGCUCUuauuuGCGuA | GuuuUAGAGCUAUGCU |
C2 | ggugagCUCUuauuugcguA | GuuuuaGAGCUAUGCU |
C3 | ggugagCUCUuauuugcguA | GuuuuaGAGCUAUGCU |
C4 | GGUGAGCUCUuauuugcguA | GuuuuaGAGCUAUGCU |
C5 | ggugagCUCUuauuugCGuA | GuuuuaGAGCUAUGCU |
C6 | ggugagCUCUuauuugcguA | GuuuUAGAGCUAUGCU |
C7 | GGUGAGCUCUuauuugCGuA | GuuuuaGAGCUAUGCU |
C8 | GGUGAGCUCUuauuugcguA | GuuuUAGAGCUAUGCU |
C9 | GGUGAGCUCUuauuugCGuA | G uuu UAGAGCUA UGC U |
C10 | GGUGAGCUCUUAUUugCGuA | G uuu UAGAGCUA UGC U |
C11 | GGU GAGCUCU UAUU ugcgu A | G uuu UAGAGCUA UGC U |
C17 | GGU GAGCUCU UAUU ug CG u A | G uuu U a GAGCUA UGC U |
C18 | GGU GAGCUCU UAUU ug CG u A | G uuuu AGAGCUA UGC U |
C19 | GGU GAGCUCU UAUU ug CG u A | G uuuua GAGCUA UGC U |
C20 | GGU GAGCUCU UAUU ug CG u A | G uuu UA GAGCUA UGC U |
C21 | GGU GAGCUCU UAUUUGCGU A | G UUUUA GAGCUA UGC U |
C22 | GGU GAGCUCU UAUU ug CG u A | G U u UUA GAGCUA UGC U |
