Fig. 7
From: Red blood cell-hitchhiking boosts delivery of nanocarriers to chosen organs by orders of magnitude
IA injection of RH NCs results in high NC uptake in any downstream organ. a Mice were anesthetized and had their right internal carotid artery cannulated to deliver the injectate to the brain (inset). Either free (blue) or RH (red) I-125-labeled NCs (here, nanogels (NGs)) were injected. The left panel depicts % injected dose (%ID) in the brain, while the right panel depicts brain/blood ratio. b Mice injected as in a, followed by sectioning and autoradiography of the brain. Scale bar represents 5 mm. c Mice were treated as in a, but the injectate was RH rhodamine-labeled NGs. Left panel is rhodamine signal, with more red puncta in the right (targeted) hemisphere. Right panel is rhodamine signal overlaid with DAPI signal. Scale bar represents 100 μm. d To demonstrate no gross anatomical damage done to the neurons by RH NGs, brain slices from c were H&E stained, showing no difference in morphology between the right and left hemispheres. Scale bar represents 100 μm. e Mice were treated as in a, but the arterial cannula was in the left kidney. f Mice were treated as in a, but the arterial cannula was in the common carotid artery, and the right and left sides of the “face” were harvested. Here, a hemi-“face” includes all structures on one side of the head, except for the brain. For all plots in this figure, each data point represents mean ± s.e.m (n = 3). *P < 0.05, non-paired, two-tailed t-test
