Fig. 1
From: Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins
Discovery of a new Acr. a Profiles of immunity generation (top, where n = the number of survivors screened) and ability to bypass immunity (bottom) of CRISPR-sensitive (green), CRISPR-resistant (red), and CRISPR-intermediate (ochre) phages. Log reductions from three technical replicates. b Representation of the genomic region in which acrIIA5 was found in D4276 for each phage in a. ORFs, represented by an arrow, are color-coded if their product shares >50% aa identity with another elsewhere in any of the other phages, and white if it does not. Functions are annotated for the first instance of any ortholog. “Acr?” was investigated for being downstream of a gene (pink) conserved in D4276. c Titres of a CRISPR-sensitive (green) or CRISPR-resistant (red) phage on their host or a CR1-immunized version thereof, in the presence or absence of the putative Acr. The presence of the Acr restores the titre of both phages to the levels observed in the absence of immunity. The titres are from three biological replicates, each of three technical replicates. Error bars represent standard deviation, and a star (*) indicates a significant difference from each other column in the graph, as calculated by one-way analysis of variance (ANOVA) and Tukey honest significant difference (HSD) test (p < 0.01)
