Fig. 4
From: Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins
Comparison of type II-A Acr activities. Fold restoration of phage titres on strains carrying an Acr over ones carrying only the empty vector. The size of the bar reflects how effective the Acr is at bypassing the CRISPR immunity present, and each is the average of three biological replicates, each of three technical triplicates. The error bars represent the standard deviation. The leftmost three sections (green) are the relative titres of phage 2972 when its host is not immunized, or immunized at the endogenous CR1 or CR3 locus. The rightmost two sections (orange) are the relative titres of phage p2 when its host is not immunized, or immunized using an exogenous plasmid-borne SpCas9. Each system (CR1, CR3, SpCas9) varies in effectiveness, so the threshold limits of detection for each is indicated by a dotted line
