Table 1 Summary of AcrIIA activities
From: Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins
Bacterial Assays | Human Assays | |||||
|---|---|---|---|---|---|---|
S. thermophilus DGCC7710 | S. thermophilus DGCC7710 | Lactococcus lactis MG1363 | K562 cells | K562 cells | ||
St1Cas9 (1121 aa) | St3Cas9 (1409 aa) | SpCas9 (1368 aa) | SpCas9 (1368 aa) | St1Cas9 (1121 aa) | ||
AcrIIA2 (123 aa) | L. monocytogenes prophage | − | − | + | − | − |
AcrIIA4 (87 aa) | L. monocytogenes prophage | − | − | + | +++ | − |
AcrIIA5 D1126 (140 aa) | S. thermophilus phage D1126 | +++ | +++ | +++ | ++ | +++ |
AcrIIA5 D4276 (140 aa) | S. thermophilus phage D4276 | +++ | + | +++ | ++ | ++ |
AcrIIA6 D1024 (183 aa) | S. thermophilus phage D1024 | +++ | − | − | − | +++ |
AcrIIA6 D1811 (183 aa) | S. thermophilus phage D1811 | +++ | − | − | − | ++ |
AcrIIA6 DT1 (183 aa) | S. thermophilus phage DT1 | +++ | − | − | − | +++ |
