Fig. 2
The development of iNKT cell subsets in thymic organ culture. a, b Thymic lobes from E18.5—day 0 BALB/c embryos were cultured in vitro and examined on days 4, 5, and 6. Percentages of total iNKT cells (TCRβint, αGC tetramer+) in live cells are shown (left column). Expression of transcription factors T-bet and PLZF (second column), surface markers CXCR3 and PD-1 (third and fourth columns) in total iNKT cells are shown. b Compiled data of the experiments. c Thymic lobes from day 0 Nur77gfp mice were cultured in vitro as in (a). d Total iNKT cells were separated into NK1.1+PD-1lo (NKT1-like) and NK1.1−PD-1hi (NKT2-like) populations and MFI of GFP from the two populations were plotted. e Thymic lobes from day 0 SKG and WT control mice were cultured in vitro as in (a), and expression levels of CXCR3 and PD-1 were compared. Data are representative of three independent experiments. Graphs represent mean ± SD with symbols representing individual mice. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; n.s. not significant (unpaired two-tailed Student’s t test)
