Fig. 3 | Nature Communications

Fig. 3

From: Altered thymic differentiation and modulation of arthritis by invariant NKT cells expressing mutant ZAP70

Fig. 3

NKT1 skewing phenotype in ZAP70AS mice. a, b Percentages in total iNKT cells and absolute numbers of iNKT cell subsets in ZAP70AS and control mice. c, d Expression of lineage defining transcription factors and some subset surface markers. e–h Day 0 thymic lobes from ZAP70AS mice were cultured as in Fig. 2. Different doses of the inhibitor 3-MB-PP1were added on the second day of culture, and iNKT cells were examined on day 7 for expression of the indicated proteins. Data are representative of three independent experiments. Graphs represent mean ± SD with symbols representing individual mice (a–d) or thymic lobes (e–h). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; n.s. not significant (unpaired two-tailed Student’s t test)

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