Fig. 8

Structural basis for resistance to rotavirus infection in non-secretors. P[6] VP8* in complex with H type I pentasaccharide is superimposed onto the structure of VP4 spike (PDB ID: 4V7Q) of P[3] animal strain RRV. The black arrow indicated how the glycan is extended from the C1 atom of Glc residue, and how the virus spike access host cell membrane. A close-up view of the P[6] VP8* with H-type I is shown in the inlet. The addition of a Lewis fucose via an α1,4 linkage to the GlcNAc moiety is indicated by a red hexagon, suggesting that Lewis fucose would clash with VP8*. The addition of GalNAc or Gal at the non-reducing end of HBGA is noted by a green hexagon, which is projecting away from VP8* without making steric hindrance