Fig. 1
Postnatal changes in microglial distribution in the cerebellum. a Immunofluorescent labeling for Iba1 (green) and calbindin staining (red) in lobules IV–V in the vermis of mouse cerebellum at P5, P8, P14, P21, and P60. EGL external granule cell layer, ML molecular layer, PCL Purkinje cell layer, IGL internal granule cell layer, WM white matter. Scale bars, 50 μm. b Frequency distribution histograms for the relative depth of microglia at P5 (n = 5 mice), P8–P9 (n = 5), P13–P14 (n = 4), P20–P21 (n = 4), and P60 (n = 6). The depth of microglia was normalized to the total length of the gray matter and the white matter where each microglial cell was sampled. c Postnatal changes in densities of microglia in the WM (blue) and the gray matter (orange). Numbers of mice at individual postnatal days are same as in (b). d Postnatal changes in the total length of the ML, PCL, IGL, and WM. e Frequency distribution histograms of the relative depth of microglia in the gray matter except the EGL (the ML, the PCL, and the IGL) at P8–P9 (left, n = 5 mice) and P13–P14 (right, n = 4). Note that microglia tend to aggregate beneath the part of the PCL (the area between orange broken lines) and the edge beside the WM at P8–P9; *p < 0.05, **p < 0.01. f Volume rendered image (508 × 508 μm, 450 μm depth) of the mouse cerebellum at P8 with immunofluorescent labeling of Iba1 (green) and calbindin (red). g Density of microglia across the layers (n = 3 mice). Data are presented as mean ± SEM
