Fig. 5
CF synapse elimination after P10–P12 is impaired in Csf1r-cKO mice. a (upper) Representative traces of CF-EPSCs in control (left), Iba1-KO (middle), and Csf1r-cKO (right) PCs in response to electrical stimulation with gradually increasing stimulus intensities; Vh = −10 mV. (lower) Frequency distribution histograms showing the number of discrete CF-EPSC steps in control (blue), Iba1-KO (green), and Csf1r-cKO (red) mice aged P21–P40. PCs were evenly sampled from all lobules. Data are sampled from four control, four Iba1-KO, and five Csf1r-cKO mice. CF synapse elimination was normal in Iba1-KO mice (p = 0.887, Mann–Whitney U test) but significantly impaired in Csf1r-cKO mice (p = 0.007). b Frequency distribution histograms of the number of CFs in control (blue) and Csf1r-cKO (red) mice at P60–P80 (p = 0.004). c–f Developmental course of CF synapse elimination. Frequency distribution histograms of the number of CFs at P6–P8 (c, p = 0.207), P10–P12 (d, p = 0.034), P13–P15 (e, p = 0.028), and P16–P18 (f, p = 0.030). Data were obtained from 4 to 5 control and 4 to 5 Csf1r-cKO mice at each postnatal period. Scale bars, 1 nA and 10 ms. Vh = −70 mV (c) and −10 mV (b, d–f). *p < 0.05; **p < 0.01; n.s., p > 0.05
