Fig. 7
Parallel fibers to PC synapses are normal in Csf1r-cKO mice. a (upper) PF-EPSCs recorded in a control or a Csf1r-cKO PC in response to paired stimuli with an interval of 50 ms. (lower) The paired-pulse ratio was not different between control (n = 30 cells from seven mice) and Csf1r-cKO (n = 32 cells from eight mice, p = 0.972, Mann–Whitney U test). b (upper) PF-EPSCs evoked by stimulus intensities of 10, 20, 30, 40, 50, and 60 μA in a control or a Csf1r-cKO PC. (lower) Stimulus–response curve of PF-EPSCs. The peak amplitudes of PF-EPSCs were measured and plotted against stimulus intensity. No difference is observed between control (n = 17 cells from six mice) and Csf1r-cKO (n = 14 cells from four mice, p = 0.459, two-way RM ANOVA). c (upper) Traces of mGluR1-mediated currents evoked by tetanic stimulation (five pulses at 100 Hz) with 10, 20, 30, 40, 50, and 60 μA stimulus intensities. (lower) The stimulus–response curves of mGluR1-mediated currents. These data were obtained from the same cells shown in (b). No difference was observed (p = 0.886, two-way RM ANOVA). d Miniature EPSCs (mEPSCs) recorded from a control or a Csf1r-cKO PC in the presence of 1 μM TTX and 100 μM picrotoxin. Amplitude (e, p = 0.921, t test) or frequency (f, p = 0.555, Mann–Whitney U test) did not differ between control (n = 11 cells from three mice) and Csf1r-cKO (n = 11 cells from three mice) mice. Vh = −70 mV (a–f). Electrophysiological data were obtained from PCs in lobules IV–V at P16–P18 (a–f). Scale bars, 100 pA and 20 ms (a, c), 200 pA and 10 ms (b), and 10 pA and 200 ms (d). n.s., p > 0.05. g–l Immunostaining for VGluT1 (g, j), calbindin (h, k), and merged of them (i, l). Images were taken from the ML in lobules IV–V in the vermis. Scale bars, 5 μm. PC dendrites possessed dense spines that faced VGluT1 puncta in both control and Csf1r-cKO mice. Data are presented as mean ± SEM
