Fig. 9
Administration of diazepam restores CF elimination in Csf1r-cKO mice. a–d Representative traces of mIPSCs recorded from a Csf1r-cKO PC before (a) and after (b) application of 1 μM diazepam. Vh = −70 mV. Scale bars, 30 pA and 250 ms. Mean amplitude of mIPSCs significantly increased during bath application of diazepam (c, p < 0.001, paired t test, n = 10 cells from three mice). Frequency was also significantly increased by the diazepam application (d, p = 0.006). e, f Representative traces of CF-EPSCs in Csf1r-cKO mice treated with vehicle (e) or diazepam (f); Vh = −10 mV. Scale bars, 500 pA and 10 ms. g Frequency distribution histograms showing the number of CFs innervating each PC in vehicle- and diazepam-treated Csf1r-cKO mice. Data were sampled from five vehicle-treated and five diazepam-treated mice. The distribution of the histogram of vehicle-treated Csf1r-cKO mice is virtually identical to that of naïve Csf1r-cKO mice (p = 0.540, Mann–Whitney U test). The percentage of mono-innervated PCs is significantly increased in diazepam-treated Csf1r-cKO mice (p = 0.011, Mann–Whitney U test). *p < 0.05; **p < 0.01; ***p < 0.001
