Fig. 2
From: NIR-II nanoprobes in-vivo assembly to improve image-guided surgery for metastatic ovarian cancer
In vitro assembly of NIR-II nanoprobes. Transmission electron microscope (TEM) images of as-made NaGdF4: 5% Nd@NaGdF4 nanocrystals (a), DNA (L1) modified DCNPs (b) and DNA complementary induced assembly between DCNPs-L1-FSHβ and DCNPs-L2-FSHβ in PBS (c). d The NIR-II fluorescence spectrum of DCNPs, DCNPs-L1-FSHβ, and self-assembled DCNPs. Inset, NIR-II fluorescence images of the corresponding samples. e Size distribution of DCNPs, DCNPs-L1-FSHβ, and self-assembled DCNPs determined by dynamic light scattering. f, g Photostability of DCNPs (f) and ICG (g) in a variety of biological media at 37 °C under continuous 808 nm laser exposure at a power density of 0.2 W cm−2. h NIR-II fluorescence images show complete attenuation of NIR-I light (ICG) by 3 mm, while NIR-II signals (DCNPs) are able to be detected through 8 mm of phantom tissues. Representative images are for n = 5 per group. i Signal to background ratios of DCNPs and ICG as a function of tissue phantom depth. Scale bars represent 10 nm in a, b and 50 nm in (c). Mean ± s.d. for n = 5 (*P < 0.05 vs. ICG, two-sided Student’s t test)
