Fig. 5
From: Controlled gene and drug release from a liposomal delivery platform triggered by X-ray radiation
In vitro gene silencing by X-ray triggered liposomes loaded with antisense oligonucleotide. a, b Representative confocal images of indirect immunofluorescence staining of PAC1R at different time points after cells were treated with (a) X-ray-triggered liposomes and (b) liposomes alone. The concentration of liposomes incubated with cells was 25 µM. Scale bar was 75 µm. Boxplots in c, d show quantitative assessment of PAC1R gene silencing induced by antisense oligonucleotide released from liposomes at different time points (c) with and (d) without X-ray radiation. Decreased PAC1R fluorescence intensity was expressed as percentage of the control. The box is bounded by the first and third quartile with a horizontal line at the median and whiskers extend to 1.5 times the interquartile range. The mean value was analysed using the t test (n = 5). *** P < 0.001, compared with the control group
