Fig. 5

Functional domains of APC2 cytoskeleton interacting region. a Schematic representation of human APC2 protein truncations generated for these experiments. Table shows whether the expressed truncations are mobile, what pattern their localization follows and what region of cell they are localized to. b Representative images of area of microtubules decorated with RFP-APC2-C in Cos7 cells (red dotted line) and total area occupied by microtubules of same cell (blue dotted line) are shown. Percentage of area of microtubule bound construct (e.g., red dotted line) over total microtubule occupied area of cells (e.g., blue dotted line) was measured and used to determine affinity of the truncations for microtubules (n = 50 cells, N = 2). c Representative images of truncated proteins expressed in Cos7 cells for 24 h before fixation and staining for tubulin, actin and EB3. Graphs represent mean ± SEM. Scale bars are 10 µm