Fig. 3
NDR1 is required for antiviral innate immune response in vivo. a Eight-week-old male NDR1+/+ and NDR1−/− mice (n = 6 for each group) were intraperitoneally injected with VSV (1 × 107 pfu g−1); 12 h later, ELISA of IFNβ, IL6, and TNFα in serum was performed. All mice were housed in SPF conditions. b VSV loads in organs were determined by TCID 50 assay from NDR1+/+ and NDR1−/− mice treated as in a. c Hematoxylin and eosin staining of liver and lung sections from mice in a. Scale bar, 100 μm (left) or 50 μm (right). d Survival curve for 8-week-old male NDR1+/+ and NDR1−/− mice challenged with VSV (1 × 108 pfu g−1, i.v.) (n = 9 per group; Wilcoxon test). All mice were housed in SPF conditions. e Survival of 8-week-old male NDR1+/+ and NDR1−/− mice intravenously injected with HSV (1 × 106 pfu per mouse) (n = 10 per group; Wilcoxon test). All mice were housed in SPF conditions. f Real-time PCR analysis of HSV genomic DNA in brains of NDR1+/+ and NDR1−/− mice (n = 6 per group) infected with HSV as in e for 4 days. g ELISA of IFNβ, IL6, and TNFα in serum of NDR1+/+ and NDR1−/− mice (n = 10 per group) infected with HSV as in e for 6 h. Data are mean ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. *p < 0.05, **p < 0.01, ***p < 0.001. See also Supplementary Fig. 3
