Fig. 5
NDR1 positively regulates antiviral immune response via promoting STAT1 expression. a Real-time PCR analysis of ISGs mRNA in WT or NDR1−/− PMs transfected with vectors expressing NDR1 or its kinase-inactive mutants, followed by stimulation with IFNβ (100 IU ml−1). b, c FACS analysis of immortalized BMDMs obtained from WT and IFNα receptor-deficient mice (b) or from WT and STAT1−/− mice (c), treated with NDR1-specific or scrambled siRNA for 48 h, followed by VSV-eGFP infection. d Real-time PCR analysis of IFNβ and ISGs mRNA in BMDMs as in c. Data are mean ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. *p < 0.05, **p < 0.01, ***p < 0.001. See also Supplementary Fig. 5
