Fig. 5 | Nature Communications

Fig. 5

From: Organoid cultures recapitulate esophageal adenocarcinoma heterogeneity providing a model for clonality studies and precision therapeutics

Fig. 5

EAC-derived organoids retain intra-tumor heterogeneity. M-FISH spectral karyotype of 10 cells from organoid culture of a CAM277 and e CAM388. Each chromosome has a unique color. Organoid cultures of b, c, d CAM277 and f, g, h CAM338 were continually cultured and sequenced at different passages to study clonal dynamics. b, f Segmented Venn diagram represents clonal composition of tumor cells at each time point. The compositions of each clone at different time points are listed in Supplementary Table 2. c, g Number of total mutations and mutated cancer-related genes from individual subclones. d, h The percentage contribution for each of the 6 mutational signatures across successive passages. The passage number is depicted as the Org_P value

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