Fig. 7

Both members of miR-146 family are redundantly essential in maintaining normal Tfh cell responses. FACS analyses and frequencies of a CXCR5⁺PD-1⁺ Tfh cells or b PNA⁺GL7⁺ GC B cells from spleens of ~8 wks old T-DKO mice or their WT littermates. FACS analyses and frequencies of c CXCR5⁺PD-1⁺ Tfh cells or d PNA⁺GL7⁺ GC B cells from spleens of ~8 wks old T-DKO mice or their WT littermates 14 days after SRBC immunization. e Fold changes (on the basis of corresponding WT controls) of serum SRBC-specific IgG levels from T-KO and T-DKO mice. f FACS analyses of the expression of ICOS in CD44^hiCD4⁺ T cells in spleen from T-KO and T-DKO mice compared to their corresponding WT littermates. Fold changes (on the basis of corresponding WT controls) of ICOS mean fluorescence intensity (MFI) were shown in the right panels. Data represent the mean ± SD and are representative of three independent experiments. Each symbol represents an individual mouse, and the bar represents the mean. Significance was determined by unpaired Student’s t-test with a 95% confidence interval. *p < 0.05