Fig. 1
From: HIV-1 targets L-selectin for adhesion and induces its shedding for viral release
L-selectin binds to HIV envelope gp120. a Blank subtracted sensorgrams of serially diluted recombinant gp120BAL binding to immobilized CD62L-Fc. b Binding of glycosylated and deglycosylated gp120 at ~2 µM concentration to immobilized CD62L-Fc and DCSIGN-Fc. The BIAcore bindings were performed as triplicates using PNGase F treated and untreated gp120s at equal concentrations. Deglycosylated gp120BAL and gp120SF33 bound significantly less to both CD62L and DC-SIGN compared to their glycosylated gp120. c ELISA binding of CD62L-Fc to immobilized gp120 in the presence of 10 mM lactose, GlcNAc (N-acetyl-d-glucosamine), sialyl-lactose, sialyl-Lewis x (sLex), fucoidan, heparin, and EDTA. sLex, fucoidan, and heparin are known ligands of CD62L and inhibited gp120 binding. The p values are calculated with respect to PBS control. d Number of gp120-QDots bound to untransfected or L-selectin-transfected HeLa cells and counted using TIRF microscopy. e Flow cytometry analysis of gp120-QDots binding to PBMC in the presence and absence of anti-CD4 (RPA-T4), and anti-CD62L (DREG-56) antibodies. f Capture of HIV-1BAL virus (1:1000 dilution) with plate-immobilized 10 µg soluble CD62L in the presence and absence of 5 mM EDTA, CD4, BSA, IgG or blank (PBS control). The captured virus was quantified using p24 ELISA (PerkinElmer). g The binding of HIV-1BAL virus (1:1000 dilution) to 106 CD62L-transfected or untransfected 293T cells. h The binding of HIV-1BAL virus to 106 sorted CD62L+ or CD62L− CD8+ T cells. The error bars indicate standard deviations from the means. The p values of all figures are calculated using unpaired Student's t test with *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. The results included in panels a−d, f−h are from at least two independent experiments with all data included
