Fig. 2
From: HIV-1 targets L-selectin for adhesion and induces its shedding for viral release
L-selectin facilitates HIV-1 infection of CD4+ T cells. a HIV infection of CD62L-transfected clones (CD62L-CEM #2 and #25) or untransfected Rev-CEM cells, as well as CD62L knockdown clones, CD62L KD #3, #8, and #32. CD62L KD #8 and #32 lost CD62L expression whereas #3 retained parental CD62L expression (Supplementary Figure 4). b HIV infection of CEM cell lines in the presence and absence of 1 µg/mL polybrene. c HIV-1BAL infection of PBMC in the presence of EDTA or anti-CD4 (RPA-T4). The infection was measured by intracellular p24+ staining. d Dose-dependent HIV-1BAL infection of PBMC in the presence of CD62L blocking antibody DREG-56 or isotype controls. Infections were measured as copy numbers of viral DNA by real-time PCR and displayed as % relative to the isotype controls. The results are from at least two independent experiments and statistics are performed without data rejections
