Fig. 3
From: HIV-1 targets L-selectin for adhesion and induces its shedding for viral release
L-selectin contributes to HIV-1 adhesion and entry. a Infection of PBMC with JRFL and SF33-pseudotyped viruses in the presence of anti-CD62L (DREG-56), anti-CD4 (RPA-T4) blocking antibodies or isotype controls. b, c Effect of L-selectin blockade in pseudovirus infections. PBMC were infected with serial dilutions of JRFL (b), SF33 (c) viruses in the presence of DREG-56 or isotype controls. Infections were measured by luciferase activities on day 3 p.i. d Infection of PBMC with equal amount of JRFL- and SF33-pseudotyped viruses produced in either 293T or 293S GnTI- cells. e The infection of PBMC by PNGase F- or mock-treated HIV-1BAL viruses. The infection level was measured as copies of HIV-1 DNA per 106 cells by real-time PCR. The results are from two independent experiments with all data included in statistical analysis
