Fig. 4
From: HIV-1 targets L-selectin for adhesion and induces its shedding for viral release
HIV-1 infection resulted in L-selectin shedding. a PBMC were infected with HIV-1BAL and CD4 and CD62L expression was measured on days 6 and 11 p.i. for p24+, p24−, and uninfected cells. The results are the representative of three individual experiments. b Histograms showing the expression of CD4, CD62L on days 6 and 11 p.i. for p24+ (solid blue), p24− (dotted), and uninfected (shaded) cells. c CCR7 and CD27 expression as defined in b. d Downregulation of CD4 and CD62L in infected PBMC is evident from the decrease in % of CD4+/CD62L+ cells and the increase in CD4-/CD62L− cells on day 6 and further on day 11 in p24+ compared to p24− and uninfected populations. e The accumulation of soluble L-selectin in the supernatant of infected and uninfected samples was measured by ELISA on days 3, 6, and 7 post HIV-1BAL infection of PBMC (Supplementary Figure 5A−C). f HIV-1 infection of PBMC induced downregulation of L-selectin expression. The loss of CD62L+ CD4 T cells during HIV-1BAL infection (upper panel) is accompanied by the increase in the viral infection (lower panel) at days 6 and 11 p.i. The results are from at least two independent experiments with all data included in the analysis
