Fig. 5

HIV-induced L-selectin shedding contributed to a loss of central memory CD4⁺ T cells. a A representative analysis of memory CD3⁺ T cells (CD45RO⁺) gated for CD27 vs. CD62L on days 6 and 11 p.i. for p24⁺, p24⁻, and uninfected populations. b Percentage of memory subset (T_CM, T_TM, and T_EM) populations for p24⁺, p24⁻, and uninfected cells on days 6 and 11. The loss of T_CM and gain of T_TM populations in infected PBMC are significant when comparing p24⁺ cells with p24⁻ and uninfected cells. In contrast, % of T_EM cells do not differ significantly among p24⁺, p24⁻, and uninfected cells. c Percentage of interferon-γ-expressing T_CM and T_EM cells in overall HIV_BAL infected and uninfected PBMC as well as p24⁺ and p24⁻ populations on day 6 p.i. d IFN-γ-, TNF-α-, and MIP-1β-expressing (%) T_CM (left panel) and T_EM (right panel) cells in p24⁺ and p24⁻ populations on day 6 p.i. The results are from at least two independent experiments with all data included in the analysis