Fig. 3
From: Molecular architecture of fungal cell walls revealed by solid-state NMR
MAS-DNP solid-state NMR reveals the tight packing of chitin and α-1,3-glucan. a 15 ms 13C–13C PAR spectrum (black) reports many intermolecular cross peaks, mainly between chitin and α-1,3-glucans. A 100-ms DARR spectrum (orange) that primarily detects intramolecular correlations is overlaid for comparison. b Summary of 65 long-range restraints. For each category, the number of all cross peaks and the number of strong and intermediate restraints (in parenthesis) are listed. c Sensitivity enhancement εon/off of 30-fold was obtained for A. fumigatus. A picture of a DNP sample is also included. d DNP-assisted intermolecular-only 15N–13C correlation spectrum unambiguously detected several chitin–glucan cross peaks. e DNP-assisted chitin-edited spectrum only shows signals from chitin itself or the glucans that are spatially proximal. The DNP experiments were conducted on a 600 MHz/395 GHz spectrometer
