Fig. 3
Global gene expression profiles of iNSCs, control NSCs, and MEFs. a–c Pairwise scatter plot analysis of the global gene expression profiles of miNSC10, SCR029, and MEF cells. The transcriptome of each cell type was profiled by RNA-seq analysis. Gene expression levels (FPKM) are depicted in log10 scale. Pearson’s correlation coefficients (r) are indicated. d Heat map of the z-transformed gene expression values in miNSC10, SCR029, and MEF cells. miNSC10 and SCR029 cells are classified into the same hierarchical cluster. e Volcano plot (significance vs. fold change) of significantly altered genes (fold change ≥ 2 and FDR < 0.05) between miNSC10 and MEF cells. f Gene ontology (GO) enrichment analysis of the upregulated genes between miNSC10 and MEF cells. The upregulated genes were analyzed for GO term enrichment by gene-set enrichment analysis (GSEA). The result was visualized on a network of gene sets (nodes) connected by their similarity (edges). Node size represents the gene-set size and edge thickness represents the degree of overlap between two gene sets. Depicted are the two major groups of enriched gene sets
