Table 1 Summary of embryo development and base editing rate using BE3 system

From: Highly efficient RNA-guided base editing in rabbit

    

Mutant ratio (%)

Target gene

No. of zygotes

No. of 2-cell (%)a

No. of blastocysts (%)a

No. of mutantsb

No. of targeted mutantsb

No. of proximal off-targetsb

No. of indelsb

No. of non-C-to-Tb

Mstn

31

29(94)

23(74)

20(87)

20(87)

4(17)

0(0)

0(0)

Dmd

23

19(83)

15(65)

12(80)

8(53)

1(7)

3(20)

1(7)

Tia1-1

25

22(88)

18(72)

15(83)

15(83)

8(44)

0(0)

0(0)

Tia1-2

22

20(91)

16(72)

12(75)

9(56)

1(6)

2(13)

1(6)

Average

 

(89.0 ± 4.6)

(70.7 ± 3.9)

(81.2 ± 5.0)

(69.7 ± 17.7)

(18.5 ± 17.7)

(8.2 ± 9.9)

(3.2 ± 3.7)

  1. aCalculated from the number of zygotes
  2. bCalculated from the number of blastocysts
Back to article page