Table 2 Generation of base conversions in Founder rabbits using BE3 and ABE7.10 system
Mutant ratio (%) | ||||||||
|---|---|---|---|---|---|---|---|---|
System | Target gene | No.of offspring | No. of mutants | No. of target mutants | No. of homozygous target mutants | No. of proximal off-targets | No. of non-C-to-T/ A-to-G | No. of indels |
BE3 | Mstn | 7 | 6(86) | 6(86) | 4(57) | 2(29) | 0(0) | 0(0) |
BE3 | Tyr | 7 | 6(86) | 6(86) | 0(0) | 6(86) | 1(14) | 1(14) |
BE3 | Lmna | 8 | 7(88) | 7(88) | 0(0) | 7(88) | 0(0) | 0(0) |
ABE7.10 | Dmd | 7 | 6(86) | 6(86) | 0(0) | 1(14) | 0(0) | 0(0) |
Average | (86.5 ± 1.0) | (86.5 ± 1.0) | (14.2 ± 28.5) | (54.2 ± 38.3) | (3.5 ± 7.0) | (3.5 ± 7.0) | ||
