Table 3 BE4-Gam system improves product purity in rabbit blastocysts

From: Highly efficient RNA-guided base editing in rabbit

    

Mutant ratio (%)

Gene

System

No. of 2-cell(%)a

No. of blastocysts(%)a

No. of mutantsb

No. of target mutantsb

No. of proximal off-targetsb

No. of indelsb

No. of non-C-to-Tb

Dmd

BE3

(89.3 ± 1.1)c

(79.3 ± 5.1)c

(82.3 ± 4.5)c

(52.3 ± 6.8)c

(8.6 ± 2.8)c

(21.6 ± 2.0)c

(8.6 ± 2.8)c

 

BE4-Gam

(89.6 ± 4.5)c

(79.3 ± 1.1)c

(85.0 ± 3.6)c

(80.3 ± 1.1)d

(4.0 ± 3.4)c

(4.3 ± 3.7)d

(0.0 ± 0.0)d

Tia1-2

BE3

(83.6 ± 3.2)c

(75.6 ± 1.1)c

(80.6 ± 1.1)c

(46.3 ± 0.5)c

(21.3 ± 2.3)c

(25.3 ± 4.7)c

(8.6 ± 3.7)c

 

BE4-Gam

(85.3 ± 5.0)c

(77.6 ± 6.4)c

(79.0 ± 2.6)c

(75.0 ± 5.2)d

(21.3 ± 8.1)c

(4.3 ± 3.7)d

(0.0 ± 0.0)d

  1. aCalculated from the number of zygotes
  2. bCalculated from the number of blastocysts
  3. c,dValues with different superscripts within the same column denote significant difference
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