Table 4 Summary of embryo development and base editing rate using ABE7.10 system

From: Highly efficient RNA-guided base editing in rabbit

    

Mutant ratio (%)

Target gene

No. of zygotes

No. of 2-cell(%)a

No. of blastocysts(%)a

No. of mutantsb

No. of target mutantsb

No. of proximal off-targetsb

No. of indelsb

No. of non-A-to-Gb

Dmd

18

15(83)

11(61)

9(82)

9(82)

0(0)

0(0)

0(0)

Otc-1

15

13(87)

9(60)

8(89)

8(89)

0(0)

0(0)

0(0)

Otc-2

14

11(79)

9(64)

6(67)

6(67)

2(22)

0(0)

0(0)

Sod1-1

15

12(80)

10(67)

10(100)

10(100)

0(0)

0(0)

0(0)

Sod1-2

16

12(75)

9(56)

4(44)

4(44)

1(11)

0(0)

0(0)

Average

 

(80.8 ± 4.4)

(61.6 ± 4.1)

(76.4 ± 21.7)

(76.4 ± 21.7)

(6.6 ± 9.8)

(0.0 ± 0.0)

(0.0 ± 0.0)

  1. aCalculated from the number of zygotes
  2. bCalculated from the number of blastocysts
Back to article page