Fig. 3
SUMO2-PCNA reduces RNAPII occupancy on chromatin. a Western blot analysis of immunopurified RNAPIIo using an α-RNAPII phosphor-CTD 4H8 antibody from formaldehyde-treated HEK293T cells overexpressing FLAG-PCNA WT, KR, or S2-KR fusion proteins. The blot was probed using an α-RNAPII A10 antibody. b, c (top) Schematic diagrams of regions containing DNA breaks associated with the FRA7K and FRA16D CFSs (light gray) in IMMP2L (b) and WWOX (c), respectively28,29. (bottom) ChIP analysis of RNAPIIo occupancy at the indicated regions of the IMMP2L (b) and WWOX (c) genes using primers derived from Helmrich et al3 (Supplementary Table 2). Each value represents the average value ± standard deviation calculated from triplicate qPCR reactions per one representative experiment. p values were calculated using t-test analysis for statistically significant differences. Only p values equal to or less than 0.05 are shown. The result was reproduced in three independent assays
