Fig. 6
Thrombin C-terminal peptides interaction and binding to human CD14 ligand binding pocket. a Ribbon representation of CD14 N-terminal hydrophobic pocket rim highlighting side-chains of residues contacting LPS (cyan), HVF18 (yellow), and both LPS and HVF18 (magenta) in an all-atom molecular dynamics simulation model. b Ribbon representation of HVF18 indicating residues in contact with CD14 hydrophobic pocket (magenta) and solvent exposed (grey). c Microscale thermophoresis assay quantifying CD14 interaction with TCPs and LPS. The peptide IVE25 was used as negative control. MST analysis was performed in concentration range 1000–0.03 μM of HVF18, GKY25, IVE25 and 500–0.03 μM of LPS. Kd constants of HVF18 = 1.2 μM, GKY25 = 6.0 μM, VFR12 = 16.8 μM and LPS = 2.1 μM were determined. The results are mean values ± SD of six measurements
