Fig. 5
From: A potentially abundant junctional RNA motif stabilized by m6A and Mg2+
m6A antibodies recognize m6A in B5ʹ but not D. Dot blot assays assessing the antibody specificity for m6A in different secondary structure contexts. Increasing amounts of 5ʹfluorescein labeled RNAs with and without m6A were spotted onto the membrane. The quantity of RNA was visualized by the fluorescein signal. Three different m6A antibodies (SySy polyclonal, NEB monoclonal, Abcam polyclonal) were tested. Red dots in the schematic secondary structures represent the m6A sites. The SS construct used in dot blot assays is 5ʹ-GGCGGm6ACUC-3ʹ. Blots shown are representative of results from three experiments
