Fig. 1
From: Dissecting myosin-5B mechanosensitivity and calcium regulation at the single molecule level
Processive movement of myosin-5B on F-actin in the single-molecule in vitro motility assay. a Schematic of the myosin-5B single molecule in vitro motility assay. A single biotinilated myosin-5B conjugated with a streptavidinated quantum dot (strep-QD) interacts with a single-actin filament. Actin filaments are anchored to the coverslip surface through N-ethylmaleimide modified myosin II (NEM). Schematic not drawn to scale. b Three consecutive frames (5 s interval) of a processive run of QD-labeled myosin-5B (red) on an actin filament (green). The yellow arrow indicates the QD position. Scale bar is 2 µm
