Fig. 1
From: FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence
Aneuploidy and mitotic duration increase during normative aging. a Aneusomy index of three chromosome pairs (7, 12, and 18) in interphase cells from different age donors. b Percentage of cytochalasin D-induced binucleated cells with chromosome 7, 12, and 18 mis-segregation. Representative images of euploid/2N and aneuploid/2N ± l cells are shown in a, b, with chromosome-specific centromeric probes as indicated. Scale bars, 5 µm. c Frame series of time-lapse phase-contrast movies of mitotic neonatal and elderly cells ± Mps1 inhibitor. Time, min:s. Scale bar, 20 µm. d Mitotic duration of individual fibroblasts from different donors, measured from nuclear envelope breakdown (NEB) to anaphase onset (ANA). e Mitotic duration under standard conditions as in d (white bars) and following treatment with Mps1 inhibitor (gray bars). Values are mean ± SD from at least three independent experiments, using two biological samples of similar age (except for progeria). Sample size (n) is indicated in each graph. NS, p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001 in comparison with neonatal (N/N) by two-tailed χ2 (a, b) and Mann–Whitney (d, e) statistical tests
