Fig. 2
From: FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence
Mitotic defects associated with advanced age. a–d Frame series of spinning-disk confocal movies of neonatal (HDF N) and elderly (HDF 87 y) dividing cells expressing H2B–GFP/α-Tubulin–mCherry. a Neonatal cell division. b–d Elderly cell with chromosome alignment delay (b) with anaphase lagging chromosome and micronucleus formation (*) (c) or with spindle mispositioning in relation to the growth surface and asynchronous adherence of the daughter cells (d) (Supplementary Movies 1–4). Time, min:s. Scale bar, 5 µm. e Mitotic duration (NEB to anaphase onset) of HDFs from different age donors imaged under spinning-disk confocal microscopy. Mitotic phenotypes are indicated by distinct colors. f Quantification of the observed mitotic phenotypes. g Percentage of cells with micronuclei in fixed-cell analysis. h Rate of cytokinesis failure under phase-contrast microscopy. Values are mean ± SD from at least two independent experiments, using two biological samples of similar age (except for progeria). Sample size (n) is indicated in each graph. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001 in comparison with N/N by two-tailed Mann–Whitney (e) and χ2 (g, h) statistical tests
