Fig. 4
From: FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence
Aging cells express low levels of FoxM1 and mitotic transcripts during mitosis. a Regulation of G2/M cell cycle genes by competitive binding of DREAM and FoxM1-MMB transcriptional complexes to CDE/CHR promoter elements, as described in ref. 40. b Venn diagram displaying the overlap between mitosis genes altered in elderly fibroblasts (87 y) and targets of DREAM/FoxM1-MMB complex40 (Supplementary Data 6). c, d FoxM1 protein levels in mitotic extracts of different age samples. FoxM1 levels were normalized to α-tubulin levels and to the neonatal sample (N/N). e FOXM1 transcript levels in mitotic total RNA from different age samples normalized to TBP transcript levels and compared to neonatal. f, g Protein levels of FoxM1 transcriptional targets in mitotic extracts of neonatal and elderly fibroblasts. Values are normalized to α-tubulin levels and to the neonatal sample (N/N). h–j Quantitative analysis of FoxM1 (h), Cyclin B (i), and Cdc20 (j) immunofluorescence intensity levels in n = single mitotic cells (N/N and 84/87 y) (Supplementary Fig. 5). Relative intensity levels are indicated as arbitrary units (A.U.). Values are mean ± SD of two (h–j) or three (d, e, g) independent experiments. NS, p > 0.05, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001 in comparison with N/N by two-tailed Mann–Whitney test
