Fig. 5
From: FoxM1 repression during human aging leads to mitotic decline and aneuploidy-driven full senescence
FoxM1 repression dictates cellular phenotypes associated with aging. a Heatmap of genes within the “mitotis” GO term differentially expressed between control and FoxM1 siRNA-depleted 10-year-old fibroblasts (2logFC cutoff value < −0.5 and > 0.5, p-value < 0.05, FDR < 5%, Supplementary Data 8). b Venn diagram illustrating the overlap between genes altered in 87 y and FoxM1 siRNA-depleted fibroblasts and the top ten altered GO terms (Supplementary Data 9). c Mitotic duration (NEB-anaphase onset) of control and FoxM1 siRNA-depleted fibroblasts. d Aneusomy index in interphase FISH. e Chromosome mis-segregation rate in CytoD-FISH. f, g Percentage of cells staining positive for the senescence markers β-galactosidase (f) and 53BP1/p21 (g). h Heatmap of SASP and senescence (CDKN1A, CDKN2A, GLB1, LMNB1) genes differentially expressed in HDF 10 y and 10 y siFoxM1 (Supplementary Data 10)36, 37. i Heatmap of senescence core signature genes differentially expressed in HDF 10 y and 10 y siFoxM1 (Supplementary Data 11); 2logFCs in red indicate genes behaving differently from expected37. In all heatmaps: p-value < 0.05; genes represented in columns and technical replicates represented in rows; Z-score column color intensities representing higher (red) to lower (blue) expression. Values are mean ± SD of three independent experiments. Sample size (n) is indicated in each graph. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, and ****p ≤ 0.0001 by two-tailed Mann–Whitney (c) and χ2 (d–g) statistical tests
