Fig. 5
From: Systems analysis of intracellular pH vulnerabilities for cancer therapy
Anti-Warburg effects of lowering pHi and inhibition of pHi-dependent metabolic targets. Seahorse flux experiments were preformed for MCF7 naïve cells under normoxia, with (low pHi, yellow grid) and without (physiological pHi) inhibition of NHE1 and of the indicated metabolic targets. The reduction in pHi (black bars) has an anti-Warburg effect on cancer cell metabolism, as measured by the ratio between the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR). Both at physiological pHi and at low pHi the additional inhibition of the selected targets (colors), amplifies the anti-Warburg effect on cancer cells. Highest OCR/ECAR ratios are obtained at low pHi (yellow grid). Seahorse experiments were done in six replicas each time and experiments were repeated three times. The bars depict the mean and the error bars depict the standard deviation of the mean
