Fig. 5
From: Accelerated microfluidic native chemical ligation at difficult amino acids toward cyclic peptides
Expanding the strategy to antimicrobial peptide RTD-1. a Three different junctions were assessed for the synthesis of RTD-1. The formation of intermediate thiolactone species imposed some adaptation to the optimal conditions defined in Fig. 4a: the residence time and temperature in µF2 were increased from 4 to 15 min and from 37 to 45 °C. b Representative HPLC chromatogram for RTD-1 synthesis from peptide 4n. c Antibacterial activity of folded RTD-1 produced from 4m against E. coli BW 25113 (filled triangles) and S. aureus SH 1000 strains (open triangles). The experiment was done in triplicate. The data correspond to the mean and standard deviation of the log10 transformed colony-forming units (cfu) per mL. The limit of detection is 100 cfu mL−1
