Fig. 2
From: Lipid droplet and peroxisome biogenesis occur at the same ER subdomains
Pex30 subdomains associate with LD biogenesis proteins. a–c Wild-type cells expressing endogenously tagged Pex30-2xmCherry and Sei1-GFP (a), Sec13-GFP (b), or Nem1-GFP (c) were grown in SC to early stationary growth phase and visualized live. Arrows indicate sites where Pex30-2xmCherry associates with other markers. Graphs to right of a and c show signal intensity on white line. d Pearson’s coefficient for colocalization of Pex30-2xmCherry punctae associated with Nem1-GFP, Sei1-GFP, and Sec13-GFP; n ≥ 50 cells. The box covers the 25th to 75th percentiles, the whiskers mark the highest and lowest values and the median is indicated. e Wild-type cells expressing endogenously tagged Pex30-2xmCherry and Sei1-GFP and Erg6-BFP expressed from a 2 μ plasmid were grown in SC to early stationary growth phase and visualized live. Arrow indicates site where Pex30-2xmCherry associates with other markers. f Quantification of experiment shown in e. Values are mean +/– s.d. of three independent experiments. g Wild-type cells expressing endogenously tagged Pex30-2xmCherry and Erg6-GFP were grown in SC to early stationary growth phase and visualized live. Arrows indicate sites where Pex30-2xmCherry and Erg6-GFP associate. Bars = 3 μm. In a–c and g stacks of three images with a step size of 0.25 μm were deconvolved; images from a single plane are shown
