Fig. 6
From: Lipid droplet and peroxisome biogenesis occur at the same ER subdomains
Human MCTP2 and C. elegans MCTP play a role in LD biogenesis. a Knockdown of expression of expression of MCTP2 and Seipin genes in HeLa cells quantified by real-time PCR (mean +/– s.d, n = 4 from two independent experiments). b HeLa cells stained with BODIPY after incubation with siRNA. Bars = 5 μm. c Quantification of diameter of LDs (μm). mean +/– s.d, from two independent experiments. **** p < 0.0001. The box covers the 25th to 75th percentiles, the whiskers mark the highest and lowest values and the median is indicated. (d) BODIPY staining of WT and mctp-1(av112) worms. Images are collapsed Z stacks by maximum projection. e–g Quantification of LD size (e), LD number (f), and embryo viability (g) of WT and mctp-1(av112) animals; * = p = 0.0326 and **** = p < 0.0001. For e and f, the box covers the 25th to 75th percentiles, the whiskers mark the 5th and 95th percentiles, the median is indicated, and the measurement from individual worms are overlaid as open circles
